Diallyl Trisulfide (H2S donor) 二烯丙基三硫

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描述

Diallyl Trisulfide (H2S donor) 二烯丙基三硫

产品标签

Diallyl Trisulfide (H2S donor) 二烯丙基三硫;GYY4137;AOAA Hemihydrochloride 氨氧基乙酸半盐酸盐;WSP-1 (Washington State Probe-1);WSP-5;CBS抑制剂;CAS NO. 2050-87-5;

产品信息

产品名称

产品编号 规格           价格(元)
Diallyl Trisulfide (H2S donor)二烯丙基三硫 MX5321-25MG 25mg

523

Diallyl Trisulfide (H2S donor)二烯丙基三硫 MX5321-100MG 100mg

1893

产品描述

二烯丙基三硫(Diallyl Trisulfide, DATS)是一种大蒜中发现的有机多硫化物,用作一种天然的硫化氢(H2S)供体[1]。DATS能够减少前列腺癌细胞PC-3的存活率(IC50=22 μM)[2]和抑制人结肠腺癌细胞HCT15的生长(IC50=11.5μM)[3]。体内,DATS阻抑前列腺癌PC-3细胞裸鼠移植瘤生长,诱导血管平滑肌松弛[4]。

产品特性

CAS NO.:2050-87-5 化学名:di-2-propen-1-yl trisulfide
同义名:DATS; NSC 651936;二烯丙基三硫醚;二烯丙基三硫化物;
分子式:C6H10S3 分子量:178.34
外观:液体 纯度:≥95%
溶解性:溶于DMSO(≥10mg/ml)、乙醇(≥5mg/ml)、不溶于水
化学结构式:

保存与运输方法

保存:-20ºC保存,至少1年稳定。

运输:冰袋运输。

注意事项

1)   本品并非商业化的临床药物,仅用作科研用途,不得用作临床诊断或治疗,不得用于食品或药品,绝对禁止用在人身上。

2)   为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用方法【源自文献,仅作参考】

文献1,Jiang, Xy., Zhu, Xs., Xu, Hy. et al. Diallyl trisulfide suppresses tumor growth through the attenuation of Nrf2/Akt and activation of p38/JNK and potentiates cisplatin efficacy in gastric cancer treatment. Acta Pharmacol Sin 38, 1048–1058 (2017). https://doi.org/10.1038/aps.2016.176

体外研究(In Vitro Assay):

细胞类型(Cell type):BGC-823 and GSE-1 cell

实验方法(Cell viability assay):The sulforhodamine B (SRB) assay was performed as described to measure BGC-823 and GSE-1 cell viability after DATS treatment. Briefly, 3.0×103 cells/well were grown in 96-well plates for 12 h and exposed todifferent concentrations of DATS (0–400 μmol/L) for 24 or 48 h.

体内研究(In Vivo Assay):

动物模型(Animal Model):Tumor xenograft mice model

实验方法(Assay):To establish gastric carcinoma xenograft tumors in mice, BGC-823 cells (5.0×106) were suspended in 100 μL PBS and subcutaneously injected into the mice. The mice were sacrificed a month later. The tumor was separated by 2 mm fragments and implanted into other mice. When tumor volumes reached approximately 100 mm3,mice were randomized and assigned to the following treatments: control (normal saline, containing 20% β-cycloamylose, every day); cisplatin (5 mg/kg, positive control, every 5 d); treated groups (DATS was formed with β-cycloamylose and dissolved in normal saline, dosage at 20, 30 or 40 mg·kg−1·d−1); and the co-treated group (cisplatin, 5 mg/kg every 5 d and DATS at 30 mg/kg all other days). All mice were sacrificed on the 32nd day,and the tumors were excised for weight measurement and histopathological analysis.

文献2,Predmore BL, Kondo K, Bhushan S, Zlatopolsky MA, King AL, Aragon JP, Grinsfelder DB, Condit ME, Lefer DJ. The polysulfide diallyl trisulfide protects the ischemic myocardium by preservation of endogenous hydrogen sulfide and increasing nitric oxide bioavailability. Am J Physiol Heart Circ Physiol. 2012 Jun 1;302(11):H2410-8. doi: 10.1152/ajpheart.00044.2012. Epub 2012 Mar 30. PMID: 22467307; PMCID: PMC3378306.

体内研究(In Vivo Assay):

动物模型(Animal Model):Tumor xenograft mice model

配制方法(Formulation):DATS was maintained in sealed amber glass ampules and kept at−20°C until use. On the day of experimentation, a fresh glass ampule of DATS was opened. DATS (5μl) was diluted in 500 μl of 100% DMSO. For in vivo experiments, the DATS in 100% DMSO solution was further diluted in sterile saline to obtain the correct dosage to be delivered in a volume of 50 μl. The resulting concentration of DMSO in this dosage was 1%. Vehicle consisted of a solution of 1% DMSO in sterile saline.

实验方法(Assay):DATS was administered at 200 μg/kg before reperfusion by either an intravenous injection 5 min before reperfusion or an intraperitoneal injection 22.5 min before reperfusion.After 24 h of reperfusion, the LV area at risk (AAR) and infarct size were determined by Evan’s blue and 2,3,5-tetrazolium chloride staining, as previously described.

参考文献

[1]Benavides, G.A., Squadrito, G.L., Mills, R.W., et al. Hydrogen sulfide mediates the vasoactivity of garlic. Proc. Natl. Acad. Sci. USA 104(46), 17977-17982 (2007).

[2]Xiao, D., Choi, S., Johnson, D.E., et al. Diallyl trisulfide-induced apoptosis in human prostate cancer cells involves c-jun N-terminal kinase and extracellular-signal regulated kinase-mediated phosphorylation of Bcl-2. Oncogene 23(33), 5594-5606 (2004).

[3]Hosono, T., Fukao, T., Ogihara, J., et al. Diallyl trisulfide supresses the proliferation and induces apoptosis of human colon cancer cells through oxidative modification of ß-tubulin. J. Biol. Chem. 280(50), 41487-41493 (2005).

[4]Xiao, D., Lew, K.L., Kim, Y.A., et al. Diallyl trisulfide suppresses growth of PC-3 human prostate cancer xenograft in vivo in association with bax and bak induction. Clin. Cancer Res. 12(22), 6836-6843 (2006).

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 — —Written/Edited by V. Shallan【版权归集奇生物/MKBio懋康所有】

其他信息

重量 50 克
品牌:

懋康MKBio

CAS:

2050-87-5

规格:

25mg

货期:

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