9-Amino-6-chloro-2-methoxyacridine (ACMA) 9-氨基-6-氯-2-甲氧基吖啶

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库存 9 件

SKU: MX4579-5MG 分类: 标签: ,

描述

9-Amino-6-chloro-2-methoxyacridine (ACMA)

9-氨基-6-氯-2-甲氧基吖啶

 

产品标签

9-Amino-6-chloro-2-methoxyacridine;9-氨基-6-氯-2-甲氧基吖啶;DNA intercalator DNA插入剂;核酸染色剂; Acridine Orange Hydrochloride 吖啶橙盐酸盐;CAS NO:3548-09-2;

产品信息

产品名称

产品编号 规格 CAS NO. 价格(元)

ACMA 9-氨基-6-氯-2-甲氧基吖啶

MX4579-5MG 5mg 3548-09-2

1293

ACMA 9-氨基-6-氯-2-甲氧基吖啶 MX4579-10MG 10mg 3548-09-2

2163

产品描述

9-氨基-6-氯-2-甲氧基吖啶(9-Amino-6-chloro-2-methoxyacridine, ACMA)是一种细胞通透性的荧光探针,一种DNA嵌入剂,选择性结合poly (dA-dT)序列,随着插入鸟苷荧光寿命逐渐减少。ACMA能用于DNA标记,激发/发射波长为411/475n,可采用大多数紫外光源激发,因而兼容更短和更长波长的染料。ACMA荧光依赖pH,并在形成pH梯度时淬灭,这一特征被用于基于动物和植物的研究中。ACMA也抑制胆碱酯酶活性,Ki为49nM。

产品特性

1) CAS NO:3548-09-2

2) 化学名:6-chloro-2-methoxy-9-acridinamine

3) 同义名:ACMA,NSC 15300

4) 分子式:C14H11ClN2O

5) 分子量:258.7

6) 外观:固体

7) 纯度:≥98%

8) Ex/Em:411/475nm

9) 溶解性:溶于甲醇

10) 化学结构式:

保存与运输方法

保存:-20°C避光干燥保存,2年有效。

运输:室温运输。

注意事项

1)荧光染料均存在淬灭问题,请尽量注意避光,以减缓荧光淬灭。

2)为了您的安全和健康,请穿实验服并戴一次性手套操作。

染色示例(来自文献,仅作参考)

Ref 1)Jin, R., He, S., Black, K.A. et al. Ion currents through Kir potassium channels are gated by anionic lipids. Nat Commun 13, 490 (2022). https://doi.org/10.1038/s41467-022-28148-4

Assay:Liposomal fluorimetric assay 

Method:The pH sensitive dye 9-amino-chloro-2-methoxyacridine (ACMA) was added to a final concentration of 2 μM from a freshly prepared 200 μM stock in 100% (v/v) ethanol. Fluorescence emission at 483 nm (excitation at 419 nm) was monitored over time with pathlengths of 2 and 10 mm for excitation and emission, respectively.

Ref 2)Furrer EM, Ronchetti MF, Verrey F, Pos KM. Functional characterization of a NapA Na(+)/H(+) antiporter from Thermus thermophilus. FEBS Lett. 2007 Feb 6;581(3):572-8. doi: 10.1016/j.febslet.2006.12.059. Epub 2007 Jan 17. PMID: 17254570.

Assay:9-Amino-6-chloro-2-methoxyacridine (ACMA) fluorescence assay to detect Na+/H+ antiport activity

Method:Na+/H+ antiporter activity was measured in a thermostated cuvette containing a continuously stirred suspension of 2 ml of 10 mM Tris–HEPES at various pH (6.0–8.0), 140 mM choline chloride, 5 mM MgCl2, 2 μM ACMA and membranes (200 μg of protein). The changes in pH inside the membranes were monitored by following the (de)quenching of ACMA fluorescence (excitation 410 nm; emission 480 nm).

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MX4579-5MG 9-Amino-6-chloro-2-methoxyacridine (ACMA) 9-氨基-6-氯-2-甲氧基吖啶 5mg

  

 — —Written/Edited by V. Shallan【版权归集奇生物/MKBio懋康所有】

其他信息

重量 50 克
品牌:

懋康MKBio

CAS:

3548-09-2

规格:

5mg

货期:

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